Journal: Journal of Cerebral Blood Flow & Metabolism

Article Title: Longitudinal imaging evaluation of the inflammatory role of purinergic A 2 A receptors during subacute and chronic ischemic stroke

doi: 10.1177/0271678X251370835

Figure Lengend Snippet: Temporal ex vivo evaluation of A 2 AR expression after cerebral ischemia. Immunofluorescent labeling of A 2 ARs (blue), NeuN (orange), CD11b (red), and DAPI (gray) in the penumbra and core of the infarction in the control and day 3 groups is shown in four channels (a–c). The data show A 2 AR expression in NeuN + and CD11b + cells from the control group (day 0) and at days 1 and 3 after cerebral ischemia in both the ischemic penumbra and the core of infarction (d, e). Immunohistochemistry labeling of NeuN and CD11b in the whole brain section before (day 0) and at days 1 and 3 after ischemia (f, g). The protein expression of A 2 ARs during the first 3 days after ischemia was evaluated by immunoblotting (h, i) and autoradiography (j, k). ** p < 0.01 and *** p < 0.001 compared with day 0; & p < 0.05 and &&& p < 0.001 compared with day 1. The values are presented as scatter dot bar plots (means ± SD). Scale bars, 30 μm (top panels) and 10 μm (bottom panels).

Article Snippet: For the first set of sections ( n = 12), the primary antibodies used were: for A 2 ARs staining, rabbit anti-rat A 2 AR (1:50; Alomone Labs, Israel); for NeuN staining, guinea pig anti-rat NeuN (1:1000; Synaptic Systems, Goettingen, Germany); and for CD11b, mouse anti-rat CD11b (1:300; Serotec, Raleigh, NC, USA).

Techniques: Ex Vivo, Expressing, Labeling, Control, Immunohistochemistry, Western Blot, Autoradiography

Journal: Journal of Cerebral Blood Flow & Metabolism

Article Title: Longitudinal imaging evaluation of the inflammatory role of purinergic A 2 A receptors during subacute and chronic ischemic stroke

doi: 10.1177/0271678X251370835

Figure Lengend Snippet: Effect of A 2 ARs on neuroinflammation after cerebral ischemia. MRI (T 2 W; a) and PET axial images of [ 18 F]DPA-714 (b) in vehicle- and CGS-treated ischemic rats at the level of the ischemic lesion. The infarct volume was evaluated via MRI (T 2 W) at day 1 after ischemia (c), and the neurological score (d) was determined at day 1 (before the start of treatment) and day 7 after MCAO in vehicle ( n = 5) and CGS-treated ( n = 5) rats. In vivo [ 18 F]DPA-714 PET signals and ex vivo immunohistochemical evaluation of TSPO expression in CD11b-positive cells were quantified on day 7 after ischemia in both the striatum and cerebral cortex (e–j). *** p < 0.001 compared with day 1, * p < 0.05 compared with the vehicle. The values are presented as violin plots showing all points (means ± SD). Scale bars, 20 μm.

Article Snippet: For the first set of sections ( n = 12), the primary antibodies used were: for A 2 ARs staining, rabbit anti-rat A 2 AR (1:50; Alomone Labs, Israel); for NeuN staining, guinea pig anti-rat NeuN (1:1000; Synaptic Systems, Goettingen, Germany); and for CD11b, mouse anti-rat CD11b (1:300; Serotec, Raleigh, NC, USA).

Techniques: In Vivo, Ex Vivo, Immunohistochemical staining, Expressing

Journal: Journal of Cerebral Blood Flow & Metabolism

Article Title: Longitudinal imaging evaluation of the inflammatory role of purinergic A 2 A receptors during subacute and chronic ischemic stroke

doi: 10.1177/0271678X251370835

Figure Lengend Snippet: Temporal ex vivo evaluation of A 2 AR expression after cerebral ischemia. Immunofluorescent labeling of A 2 ARs (blue), NeuN (orange), CD11b (red), and DAPI (gray) in the penumbra and core of the infarction in the control and day 3 groups is shown in four channels (a–c). The data show A 2 AR expression in NeuN + and CD11b + cells from the control group (day 0) and at days 1 and 3 after cerebral ischemia in both the ischemic penumbra and the core of infarction (d, e). Immunohistochemistry labeling of NeuN and CD11b in the whole brain section before (day 0) and at days 1 and 3 after ischemia (f, g). The protein expression of A 2 ARs during the first 3 days after ischemia was evaluated by immunoblotting (h, i) and autoradiography (j, k). ** p < 0.01 and *** p < 0.001 compared with day 0; & p < 0.05 and &&& p < 0.001 compared with day 1. The values are presented as scatter dot bar plots (means ± SD). Scale bars, 30 μm (top panels) and 10 μm (bottom panels).

Article Snippet: For the first set of sections ( n = 12), the primary antibodies used were: for A 2 ARs staining, rabbit anti-rat A 2 AR (1:50; Alomone Labs, Israel); for NeuN staining, guinea pig anti-rat NeuN (1:1000; Synaptic Systems, Goettingen, Germany); and for CD11b, mouse anti-rat CD11b (1:300; Serotec, Raleigh, NC, USA).

Techniques: Ex Vivo, Expressing, Labeling, Control, Immunohistochemistry, Western Blot, Autoradiography

Journal: Journal of Cerebral Blood Flow & Metabolism

Article Title: Longitudinal imaging evaluation of the inflammatory role of purinergic A 2 A receptors during subacute and chronic ischemic stroke

doi: 10.1177/0271678X251370835

Figure Lengend Snippet: Effect of A 2 ARs on neuroinflammation after cerebral ischemia. MRI (T 2 W; a) and PET axial images of [ 18 F]DPA-714 (b) in vehicle- and CGS-treated ischemic rats at the level of the ischemic lesion. The infarct volume was evaluated via MRI (T 2 W) at day 1 after ischemia (c), and the neurological score (d) was determined at day 1 (before the start of treatment) and day 7 after MCAO in vehicle ( n = 5) and CGS-treated ( n = 5) rats. In vivo [ 18 F]DPA-714 PET signals and ex vivo immunohistochemical evaluation of TSPO expression in CD11b-positive cells were quantified on day 7 after ischemia in both the striatum and cerebral cortex (e–j). *** p < 0.001 compared with day 1, * p < 0.05 compared with the vehicle. The values are presented as violin plots showing all points (means ± SD). Scale bars, 20 μm.

Article Snippet: For the first set of sections ( n = 12), the primary antibodies used were: for A 2 ARs staining, rabbit anti-rat A 2 AR (1:50; Alomone Labs, Israel); for NeuN staining, guinea pig anti-rat NeuN (1:1000; Synaptic Systems, Goettingen, Germany); and for CD11b, mouse anti-rat CD11b (1:300; Serotec, Raleigh, NC, USA).

Techniques: In Vivo, Ex Vivo, Immunohistochemical staining, Expressing